After the preliminary experiments, DTU completed a series of Adaptation Laboratory Evolution (ALE) experiments to improve the robustness of the strains Actinobacillus succinogenes 130Z and Basfia succiniciproducens. The ALE procedure significantly improved the tolerance of the evolved strains to the highly concentrated waste stream of IVEM.
Validation of the results, selection of the optimal evolved strain among all lineages generated, and further investigation of these strains is currently ongoing at DTU.
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