Figure 1. Workflow for isolation and validation of evolved succinic-acid-producing strains.
DTU is currently preparing the pipeline for genomic profiling of the mutant ALE (Adaptive laboratory Evolution) strains with superior tolerance to high substrate concentrations. A second ALE protocol to improve the tolerance to high product titers was also attempted in order to promote higher succinic acid concentrations at the end of fermentations. The second ALE protocol was completed successfully in the past months. We are currently addressing a few challenges on the isolation step to obtain highly product tolerant clones. The genomic profiling of all ALE strains generated throughout the project will be performed in the coming months.